Microtiter determination of measles hemagglutination inhibition antibody with filter papers
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چکیده
منابع مشابه
Microtiter determination of measles hemagglutination inhibition antibody with filter papers.
Epidemiological studies of measles and measles immunization frequently require determination of measles antibody status. In developing countries, where venipuncture is frequently unacceptable and where refrigerated storage of serum specimens is often unavailable, microtiter techniques not requiring refrigeration are required. We developed a filter paper technique that measures measles hemagglut...
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The rapid microtiter technique was investigated as a means of facilitating the detection of staphylococcal enterotoxin B. With this technique, many samples were assayed simultaneously, and readable results were obtained in 3 hr. Other advantages of this method, in addition to speed, were the small quantity of reactants used, ease of reading, and reproducibility.
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Measles continues to be a major disease of both human and nonhuman primates. The dot immunobinding assay, a modified enzyme immunoassay, permits the detection of measles virus antibody in the nonlaboratory setting with either serum or whole blood collected on filter paper.
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Erthrocytes collected from monkey species including grivet, rhesus, and cynomolgus monkeys were stabilized by fixation with glutaraldehyde of a low concentration and were freeze-dried in vacuo. These freeze-dried erythrocytes were compared with fresh erythrocytes for measles viral hemagglutination and hemagglutination inhibition and could be used in both tests instead of fresh erythrocytes. The...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1983
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.17.5.860-863.1983